Isolation of a DNA-RNA complex fromEuglena gracilis

Dna-dependent Rna Synthesis in Chloroplasts of Euglena Gracilis

Isolated chloroplasts of Euglena gracilis have been shown to be capable of incorporating amino acids into protein (2). This incorporation is inhibited by actinomycin D, a fact which suggests that the chloroplasts might be capable of DNAdependent RNA synthesis. Such RNA synthesis is also indicated in a recent report (6) showing inhibition of chlorophyll synthesis by actinomycin. In conjunction w...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

A rapid and efficient method for isolation of total RNA from Euglena gracilis (Euglenoidea).

RNA isolation is essential to the study of gene expression at the molecular level. However, it is difficult to isolate RNA from organisms that contain large amounts of polysaccharides or other compounds that bind or coprecipitate with RNA, such as the unicellular protist Euglena gracilis. Currently, there is no commercial kit available that is specific for the isolation of high-quality RNA from...

Selective medium for isolation of Bacteroides gracilis.

A new medium selective for Bacteroides gracilis was developed. The medium is tryptic soy agar (Difco Laboratories, Detroit, Mich.) containing nalidixic acid, teicoplanin, sodium formate, sodium fumarate, and potassium nitrate. All 18 strains of B. gracilis tested grew with only minimal inhibition. Most of the other 214 organisms tested, including most Bacteroides species, other anaerobes, and a...

Isolation of high-quality RNA from a wide range of woody plants

Isolation of high-quality RNA is one of the most crucial methods in molecular biology. RNA extraction from woody plants has been problematic due to the presence of rigid and woody tissues, large amounts of polysaccharides, polyphenols and other secondary metabolites. Here we present a suitable protocol for RNA isolation from a wide range of woody plants that includes eight gymnosperms and four ...